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htert immortalized rpe1  (ATCC)


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    Structured Review

    ATCC htert immortalized rpe1
    Htert Immortalized Rpe1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2237 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/htert+rpe1/hTERT+RPE-1/pm42265085-284-6-8
    Average 99 stars, based on 2237 article reviews
    htert immortalized rpe1 - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: The SMARCA4 subunit of the SWI/SNF complex prevents genome instability at G quadruplexes.
    Article Snippet: .. hTERT-RPE1 (RPE1, source: ATCC, not authenticated further) were cultured in Dulbecco modified minimal essential medium (DMEM)/F-12 (Merck) supplemented with 10% FBS (Gibco), 200μM glutamax (Gibco), 0.26% sodium bicarbonate (Gibco), and 1% penicillin/streptomycin (P/S; Merck). .. hTERT-RPE1 (RPE1, source: ATCC, not authenticated further) were cultured in Dulbecco modified minimal essential medium (DMEM)/F-12 (Merck) supplemented with 10% FBS (Gibco), 200μM glutamax (Gibco), 0.26% sodium bicarbonate (Gibco), and 1% penicillin/streptomycin (P/S; Merck).

    Article Title: An SP110-SP100 axis is a critical regulator of promyelocytic leukaemia body dynamics and mitotic fidelity.
    Article Snippet: Stimulation of the innate immune system by foreign RNA elicits a potent interferon response and can trigger cell death.. The mechanisms by which cells balance a robust response with cell-intrinsic lethality are still being uncovered.. Here, using genome-wide CRISPR–Cas9 genetic screens w ith t ri ph os ph or ylated RNA stimulation, we discover that promyelocytic leukaemia (PML) nuclear body-localized speckled protein 110 (SP110) is a potent inhibitor of type 1 interferon-driven cell death.

    Article Title: CENP-B binds hairpin motifs in chromosome arms influencing gene expression
    Article Snippet: .. hTERT-RPE1 were obtained from ATCC (catalogue number CRL-4000) and cultured in Dulbecco Modified Minimal Essential Medium (DMEM)/F-12 (Sigma) supplemented with 10% FBS (Gibco), 200 μM glutamax (Gibco), 0.26% sodium bicarbonate (Gibco), and 1% penicillin/streptomycin (P/S) (Sigma). .. HEK293TN cells were obtained from ATCC and cultured in DMEM supplemented with 10% FBS (Gibco), and 1% penicillin/streptomycin (P/S) (Sigma).

    Article Title: High resolution interaction surface mapping by PRISMA reveals novel ARID1A interactions
    Article Snippet: .. hTERT-RPE1 (RPE1, ATCC) were cultured in Dulbecco Modified Minimal Essential Medium (DMEM)/F-12 (Merck) supplemented with 10% FBS (Gibco), 200μM glutamax (Gibco), 0.26% sodium bicarbonate (Gibco), and 1% penicillin/streptomycin (P/S)(Merck). ..

    Modification:

    Article Title: The SMARCA4 subunit of the SWI/SNF complex prevents genome instability at G quadruplexes.
    Article Snippet: .. hTERT-RPE1 (RPE1, source: ATCC, not authenticated further) were cultured in Dulbecco modified minimal essential medium (DMEM)/F-12 (Merck) supplemented with 10% FBS (Gibco), 200μM glutamax (Gibco), 0.26% sodium bicarbonate (Gibco), and 1% penicillin/streptomycin (P/S; Merck). .. hTERT-RPE1 (RPE1, source: ATCC, not authenticated further) were cultured in Dulbecco modified minimal essential medium (DMEM)/F-12 (Merck) supplemented with 10% FBS (Gibco), 200μM glutamax (Gibco), 0.26% sodium bicarbonate (Gibco), and 1% penicillin/streptomycin (P/S; Merck).

    Article Title: An SP110-SP100 axis is a critical regulator of promyelocytic leukaemia body dynamics and mitotic fidelity.
    Article Snippet: Stimulation of the innate immune system by foreign RNA elicits a potent interferon response and can trigger cell death.. The mechanisms by which cells balance a robust response with cell-intrinsic lethality are still being uncovered.. Here, using genome-wide CRISPR–Cas9 genetic screens w ith t ri ph os ph or ylated RNA stimulation, we discover that promyelocytic leukaemia (PML) nuclear body-localized speckled protein 110 (SP110) is a potent inhibitor of type 1 interferon-driven cell death.

    Article Title: CENP-B binds hairpin motifs in chromosome arms influencing gene expression
    Article Snippet: .. hTERT-RPE1 were obtained from ATCC (catalogue number CRL-4000) and cultured in Dulbecco Modified Minimal Essential Medium (DMEM)/F-12 (Sigma) supplemented with 10% FBS (Gibco), 200 μM glutamax (Gibco), 0.26% sodium bicarbonate (Gibco), and 1% penicillin/streptomycin (P/S) (Sigma). .. HEK293TN cells were obtained from ATCC and cultured in DMEM supplemented with 10% FBS (Gibco), and 1% penicillin/streptomycin (P/S) (Sigma).

    Article Title: High resolution interaction surface mapping by PRISMA reveals novel ARID1A interactions
    Article Snippet: .. hTERT-RPE1 (RPE1, ATCC) were cultured in Dulbecco Modified Minimal Essential Medium (DMEM)/F-12 (Merck) supplemented with 10% FBS (Gibco), 200μM glutamax (Gibco), 0.26% sodium bicarbonate (Gibco), and 1% penicillin/streptomycin (P/S)(Merck). ..

    other:

    Article Title: Contractile perinuclear actomyosin network promotes peripheral and polar chromosome interaction with the mitotic spindle
    Article Snippet: 19822203 Software, algorithm Imaris, Surface cut at defined spots tool Bitplane Custom surface cut tool adopted to cut at a perpendicular at user defined spots; https://doi.org/10.5281/ zenodo.19822203 Software, algorithm Fiji https://www.fiji.sc RRID:SCR_002285 Software, algorithm Fiji, macro for tracking PANEM progress with respect to the cell center Custom made; https://doi.org/10.


    Multiple Displacement Amplification:

    Article Title: Bulky PP1 analogs exert cellular effects independently from analog-sensitive kinase inhibition
    Article Snippet: .. We grew hTERT-RPE1 (telomerase-immortalized human retinal pigment epithelium cells; ATCC CRL-4000), HaCaT (immortalized human keratinocytes; AddexBio T0020001), and MDA-MB-231 (human breast adenocarcinoma cells; ATCC HTB-26) in DMEM medium (Gibco, 61965-059) supplemented with 10% fetal bovine serum (FBS, Gibco, 10270-106) unless otherwise indicated. .. We grew THP-1 (acute monocytic leukemia cells; ATCC TIB-202) in MEM medium (Gibco, 51200-046) supplemented with 10% FBS, 1% glutamax (Gibco, 35050-061) and 1 mM sodium pyruvate (Gibco, 11360-039).



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    PP1 analogs inhibit cell migration. Cell migration of hTERT-RPE1 (left), HaCaT (middle) and MDA-MB-231 cells (right) was assessed by monitoring wound closure every 2 h over 48 h, in presence of various concentrations of PP1 inhibitors. Cytochalasin D, a potent actin polymerization inhibitor, was used as a reference inhibitor of cell migration . Relative wound cellular density was determined in 3 or 4 different wells. Histograms showing relative cellular densities after 12, 24, 48 h of treatments are shown in .

    Journal: Frontiers in Chemistry

    Article Title: Bulky PP1 analogs exert cellular effects independently from analog-sensitive kinase inhibition

    doi: 10.3389/fchem.2026.1812827

    Figure Lengend Snippet: PP1 analogs inhibit cell migration. Cell migration of hTERT-RPE1 (left), HaCaT (middle) and MDA-MB-231 cells (right) was assessed by monitoring wound closure every 2 h over 48 h, in presence of various concentrations of PP1 inhibitors. Cytochalasin D, a potent actin polymerization inhibitor, was used as a reference inhibitor of cell migration . Relative wound cellular density was determined in 3 or 4 different wells. Histograms showing relative cellular densities after 12, 24, 48 h of treatments are shown in .

    Article Snippet: We grew hTERT-RPE1 (telomerase-immortalized human retinal pigment epithelium cells; ATCC CRL-4000), HaCaT (immortalized human keratinocytes; AddexBio T0020001), and MDA-MB-231 (human breast adenocarcinoma cells; ATCC HTB-26) in DMEM medium (Gibco, 61965-059) supplemented with 10% fetal bovine serum (FBS, Gibco, 10270-106) unless otherwise indicated.

    Techniques: Migration